Comparative Researches on Two Kinds of Staining Method of Acetylcholinesterase Isozymes

摘要：

　　The staining effects of two staining methods of acetylcholinesterase isozymes were compared.One using acetylthiocholine iodide,K3Fe(CN)6 and CuSO4 was named as AFCu method,and the other one using acetylthiocholine iodide,K3Fe(CN)6 and FeCl3 was named as AFFe method.The staining effect of former was better.By physiological researches the acetylcholinesterase (AChE,EC.3.1.1.7),xisting in red cells,liver,spleen,nerve and muscle,plays an important role in erveconduction processes in lives1.For its close relationships with Alzheimers disease (AD),close attention to the function of the AChE in the formation and development of lzheimers disease2 and the screen of its inhibitors 1,3,4have been paid in the academic world.Otherwise,to meet the need of rapid detection of organophosphorus pesticides and monitoring of environmental pollution 1,5,the AChE has also become more and more important.Two staining methods of AChE isozymes will be compared,in order to be helpful to the researches on the AChE.

关键词： acetylcholinesterase isozyme staining method K3Fe(CN)6 CuSO4 FeCl3

作者: Gan Zhao Kangjie Chen Shaocong Wu Qinchuan Zhuang Fang Qian

作者单位: College of Life Science, South China Agricultural University, Guangzhou 510642, China College of Science, South China Agricultural University, Guangzhou 510642, China College of Nursing, Guangzhou Medical College, Guangzhou 510180, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 3-6

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）