Analysis of synonymous codon usage bias in D15 gene encoded surface antigen of Riemerella anatipestifer
In order to provide a basis for understanding the evolutionary relationship and pathogenesis of Riemerella anatipestifer and selecting a appropriate host expression systems to improve the expression of target gene in vivo and in vitro,we identified the codon bias in the newly confirmed D15 gene of Riemerella anatipestifer ATCC 11845 strain and performed comparative analysis of the codon usage bias between DI5 gene in R.anatipestifer and the other 10 referenced Flavobacteriaceaes by a series of online bioinformatics softwares.The results revealed that the synonymous codons with A and T at the third codon position had widely usage in the codon of D15 gene of R.anatipestifer.In addition,there were 70 rare codons in the ORF of the D15 of R.anatipestifer,and 32 codons showing distinct usage differences between R.anatipestifer and E.coli,30 codons between R.anatipestifer and Homo sapiens,16 codons between R.anatipestifer and yeast,indicated the yeast expression system may be more suitable for the expression of R.anatipestifer genes.The extent of codon usage bias in the D 15 gene in R.anatipestifer was highly correlated with the gene expression level,therefore the results may provide useful information for gene classification and functional studies.
Riemerella anatipestifer codon usage bias D15 gene
Bin Feng Dekang Zhu Xiaojia Wang Anchun Cheng Mingshu Wang
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University,46 Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city
国际会议
厦门
英文
597-605
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）