Characterization of Synonymous Codon Usage in the R1 Gene of Duck Enteritis Virus
In this paper,a corresponding analysis of the codon usage bias in the large subunit of ribonucleotide reductase (R1),encoded by UL39 gene from duck enteritis virus (DEV) CHv strain (Assigned Accession No.:EU071042) and 33 other reference herpesviruses was performed by using CAI,CHIPS and CUSP program of EMBOSS,aims to provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems.The results showed that codon usage bias of DEV R1 gene strongly preferred to the synonymous with A and T at the third codon position; the phylogentic analysis revealed that DEV had a close evolutionary relationship with the avian Alphaherpesvirinae.In addition,the codon usage bias of DEV R1 gene was compared with those of E.coli,yeast and human.There are 17 codons showing distinct usage differences between DEV and E.coli,13 codons between DEV and yeast,20 codons between DEV and human.Therefore,the yeast expression system is more suitable for the target genes expression.The extent of codon usage bias in the DEV R1 gene was highly correlated with the gene expression level,therefore the results may provide useful information for the study of classification and function of the target gene.
Duck Enteritis Virus (DEV) R1 gene codon usage bias characterization
Guofu LU Anchun CHENG Mingshu WANG
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu city
国际会议
厦门
英文
701-711
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）