Detection of L-tert-leucine in the enzyme-catalyzed reaction system

摘要：

　　Direct determination of L-ter-leucine in enzyme catalytic reaction system by HPLC was studied.The detection were performed on a Kromasil 700-5C 18 column using a eluant containing 0.25% (NH4)H2PO4 and 100% methanol (V((NH4)H2PO4)∶ V(methanol)=100∶5) with the flow rate of 0.8 ml/min at,detection wavelength of 205nm.There was a good line correlation between peak area and contents in the rang of 0.2-10 mg/ml,the correlation coefficient was 0.9986,the average recovery was 98.88% with a relative stand deviation of 0.78% (n=5).This method is simple,stable,accurate and reliable for the quality control of L-ter-leucine.

关键词： L-ter-leucine enzyme catalytic HPLC

作者: Sheng Deng Li Cui Limin Ma

作者单位: light industry and chemical engineering, Dalian Polytechnic University, Dalian 116034, P.R.China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 717-720

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）