D-lactic acid production from Xylose in engineered Escherichia coil SZ470

摘要：

　　WD100,knocked out adhE of Escherichia coli SZ470 and inserted ldhA into Escherichia coli WD01,was genetically engineered to utilize xylose.D-lactate production was investigated for shake flask cultures with xylose.In 64h WD 100 produce 10.1 g/L D-lactate in the shaking flask And it consumed 25g/L xylose during the ending of fermentation.This volumetric productivity with xylose is 0.14 g·L-1·h-1.Because of pyruvate decarboxylase (poxB) expressed in flask fermention,acetate production was up to 4.7g/Lo Succinate,formate,ethanol was also produced as a minor product during fermentation.

关键词： Lactic acid fermentation Escherichia coli xylose polylactic acid(PLA)

作者: Zhaomin Zheng Tian Tian Jinhua Wang Yongze Wang Shengde Zhou

作者单位: Key Laboratory of Fermentation Engineering(Ministry of Education), College of Bioengineering,Hubei U Key Laboratory of Fermentation Engineering(Ministry of Education), College of Bioengineering,Hubei U

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 721-724

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）