Effect of 5-fluorouracil on the regulation of zebrafish thymidylate synthase gene expression
Thymidylate synthase (TS) is an important target in cancer therapy,which is a folate-dependent enyme,catalyzing the de novo synthesis of dUMP.In this report,the effect of 5-flurorouracil (5-FU)on the regulation of TS gene expression was estimated in zebrafish.The results showed 5-FU could significantly increase the TS expression in zebrafish embryos.However,TS mRNA level were remained unchanged.To determine the effect of 5-FU and 5-FdUMP on translation of TS mRNA,a rabbit reticulocyte lysate translation system was used.Addition of 5-FU,not inhibited the translation of TS mRNA.While addition of 5-FdUMP,completely repressed the translation of TS mRNA.Therefore,induced expression of thymidylate synthase by 5-FU in zebrafish occurred in translational level,not in transcriptional level.The findings demonstrated that zebrafish TS protein was able to bind to its own cogate mRNA and the 5-FU regulated TS in the translational level.This is the first time to confirm that the regulation of TS is affected by TS and its cognant mRNA interaction in the whole animal level.
5-FU zebrafish thymidylate synthase gene expression
Chunxia Song Qian Zhang Yuliang Xiao
Marine College, Shandong University at Weihai, Weihai 264209, China College of Pharmaceutical Sciences, Taishan Medical University, Taian 271000, China
国际会议
厦门
英文
732-735
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）