Effect of Tumstatin T-7 Peptide on HepG-2 cells and Human Umbilical vein Endothelial cells

摘要：

　　The direct anti-tumor T-7 peptide of tumstatin was obtained to study its effect on human hepatoma cells (HepG-2) and human umbilical vein endothelial cells (ECV304).T-7 peptide was synthesized and its purity was up to 98.5%.MTT assay,growth curve,transmission electron microscopy(TEM) were used to detect the proliferation inhibition and pro-apoptotic function.MTT experiments and growth curve experiments showed that the survival of human hepatoma cells (HepG-2) decreased in a time-and dose-dependent way with the concentration of T-7 peptide increased.T-7 peptide can induce apoptosis of HepG-2 significantly.Apoptosis features were observed by TEM.However,the inhibition ofT-7 peptide on human umbilical vein endothelial cells (ECV304) was weaker.Experiments showed thatT-7 peptide can affect HepG-2 cells.It had a certain therapeutic effect on human hepatocellular carcinoma.T-7 peptide have little effect on ECV304 cells,which means it didnt influence the formation of tumor angiogenesis and normal ceils.

关键词： tumstatin cell proliferation cell apoptosis hepatocarcinoma angiogenesis

作者: Shujing Wang Fei Wang Jia Liu Shan Jiang Ning Chen Lin Liu Ying Zhao Xiaodan Zhang

作者单位: College of pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, China, 150076

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 744-747

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）