Expression of Aminolevulinate Dehydratase During Mycelium Development in Monascus purpureus
During the initial steps ofporphyrins biosynthesis,two molecules of 5-aminolevulinic acid are asymmetrically condensed to porphobilinogen by aminolevulinate dehydratase (ALAD).To investigate the relationship between the mycelium development and gene expression for ALAD in Monascus purpureus,the cDNA coding for ALAD of M.purpureus was isolated.The recombinant ALAD protein was expressed in E.coli BL21 (DE3) cells and subsequently purified using affinity chromatography.We analyzed the phylogenetic variation in metal ion usage by ALAD enzymes,and suggested that MpALAD utilize zinc to act in catalytic site.Transcriptional expression of MpALAD during M.purpureus liquid cultivation process was assayed by RT-PCR analysis on mycelium at two distinct development stages.With the transition of mycelium color from white to red,a decrease in the transcript level for MpALAD gene was observed.The results revealed that the transcriptional expression of MpALAD is developmentally regulated in M.purpureus.
Aminolevulinate dehydratase Monascus purpureus Mycelium development
Xiu Xue Lan Gao
School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou 510006, P.R.China
国际会议
厦门
英文
760-764
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）