Heterologous Expression of Production of T10, C12-CLA linoleic acid isomerase gene from Propionibacterium acnes

摘要：

　　The linoleic acid isomerase enzyme from Propionibacterium aches,which catalyzed the isomerization of a methylene-interrupted double bond system to a conjugated double bond system,creating trans-10,cis-12 conjugated linoleic acid (tl0,c12 CLA) was cloned and overexpressed in Escherichia coli using expression system of pCold-SUMO.The gene was expressed under T7 promotor with a fusion parmer of 6×His.Tag at its 5 terminal.After induction by 0.2mM IPTG for 18 h,the recombinant enzyme are expressing in the cytoplasm.The 1275 bp gene from P.acnes encode a linoleic acid isomerase protein with 424 amino acids (55KD).

关键词： Recombinant plasmid Linoleic acid isomerase gene Biotransformation

作者: Xue Luo LanWei Zhang HongBo Li ShuMei Wang ChaoHui Xue

作者单位: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 765-768

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）