SNP Detection by Using Affymetix Barley 1 Array in Barley
Nowadays the research for discovering SNPs is becoming one of the most important topics to understand the differences between genotypes of the same species.The objective of this study is to discover the SNPs using oligonucleotide microarrays in barley.Two methods are used for the prediction step:one is Robustified Projection Pursuit (RPP) method and another is based on a statistic test between probes ofa probeset belonging different genotypes.Following the procedure described above,124 single feature polymorphisms (SFPs),which can be used for identification of SNPs or insertion/deletions (INDELs),were detected among all genotypes.Sequencing of putative SFP-containing amplicons from the EST region in the genome yielded a validation rate of 85%,indicating the Affymetrix Barley 1 array could provide a satisfactory platform for identification of SNP in barley.
SNP detection Robustified Projection Pursuit Method Barley 1 GeneChip
Peiguo Guo Ronghua Li Nicoló Salvato Michele Filosi Yanshi Xia
College of Life Sciences, Guangzhou University, Guangzhou 51006, China College of Life Sciences, Guangzhou University, Guangzhou 51006, China ;Information Engineering Depa
国际会议
厦门
英文
803-807
2012-12-28（万方平台首次上网日期，不代表论文的发表时间）