The effect of T-7 peptide on human Non-small-cell carcinoma A549 Cells

摘要：

　　7 peptide of Tumstatin(T-7 peptide) is composed of 185-191 amino acids.To study T-7 peptide antitumor activity to human non-small-cell carcinoma(A549),T-7 peptide was designed and synthesized by amino acid synthesizer.Its purity ran up to 98.45% by HPLC and MS.The effect of T-7 peptide on A549 cell growth was observed by MTT assay,growth curve and transmission electron microscopy(TEM).T-7 peptide had the effects of suppressing A549 cell growth and promoting its apoptosis,showing dose-and time-dependent.Its IC50 was 92.84 μg/ml.TEM also revealed that A549 cell treated with T-7 peptide appeared apoptotic morphology,such as cell pyknosis and mitochondrial vacuoles formed.While T-7 peptide had little effect on human umbilical vein endothelial cells(ECV304).These researches were significant to treat human non-small-cell carcinoma in the future.

关键词： Tumstatin cell proliferation cell apoptosis non-small-cell carcinoma T-7 peptide

作者: Shujing Wang Shan Jiang Jia Liu Fei Wang Ning Chen Lin Liu Ying Zhao Xiaodan Zhang

作者单位: College of pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, China, 150076

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 820-823

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）