Thermal alkaline method to prepare Geotrichum Candidum protein foaming agent
Geotrichum Candidum strain numbered 2.0498 was used as raw material.the Geotrichum Candidum thallus were collected through inoculation and shaking cultivation.Thermal alkaline method was applied for hydrolyzing Geotrichum Candidum mycoprotein to prepare protein foaming agent.The effect of bacteria concentration,Ca(OH)2 mass concentration,hydrolysis temperature and hydrolysis time on foam expansion were studied.The optimization strategy was single factor test and subsequent response surface analysis based on Box-Behnken design.The results showed that the optimized condition were a bacteria concentration of 5.4%(w/v) and a Ca(OH)2 mass concentration of 1.5%(w/v),at a hydrolysis temperature of 95℃ for 4.1 h.Under these optimal conditions,the foam expansion was 28.1.Compared with the model theory prediction,the relative error was 0.46%.At the same time,a multiple quadratic regression equation between Geotrichum Candidum mycoprotein foam expansion and impact factors was setted up and it played an important role in predicting the foam expansion.
Geotrichum Candidum foaming agent foam expansion RSM
Jiejie Zheng Jichuan Huo Hong Lei Wei Ai
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China
国际会议
厦门
英文
845-849
2012-12-28(万方平台首次上网日期,不代表论文的发表时间)