Influence of blocking agents on non-specific background of polystyrene microbeads in serum immunoassay

摘要：

　　In this paper,we used bovine serum albumin and polymer as the blocking agents and investigated the effect of blocking agents on non-specific background of polystyrene microbead that used the human serum immunoassay.The results showed that the nonspecific background is lower by using polymer blocking agents.The best blocking condition was that microbeads were blocked by PVXT (0.5% polyvinyl alcohol PVA,0.8% polyvinylpyrrolidone,0.05% Tween-20,PBS phosphate buffer,pH7.0) for two hours at room temperature.

关键词： Polystyrene microbead Polyvinyl Alcohol Polyvinylpyrrolidone Bovine Serum Albumin(BSA)

作者: Zhimin Ren Xi Nie Shengshu Ai

作者单位: School of Water Conservation&Environment Engineering,Changchun Institute of TechnoLogy,Changchun 130012, China ;Jilin Provincial Key Laboratory of Urban Sewage Treatment, Changchun 130012, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 858-861

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）