Sample preparation for HPLC determination of free and oligosaccharides-bound sialic acid in bovine colostrum

摘要：

　　An analytical method was developed for determining free and oligosaccharide-bound sialic acids in bovine colostrum by HPLC.The condition of acid hydrolysis for sample and sialic acids derivatization were investigated.The sialic acids were released from their parent oligosaccharides by acid hydrolysis using 40mL 0.1 mol/L sulphuric acid for 100min.They were then derivatized using O-phenylenediamine (OPD) at 70℃ for 40 min,the determination of the derivative could be carded out by UV detection.The total content of free and oligosaccharide bound sialic acids in bovine colostrurn milk were 941.95μg/mL.The developed method is validated on various dairy products such as bovine milk and milk-based products.

关键词： Salic Acid Bovine Colostrum Hydrolysis HPLC

作者: Minpeng Zhu Suhong Li Xiuhong Zhao Peng Guan

作者单位: College of Grain Science and Technology, Shenyang Normal University Shenyang, Liaoning province, Chi Liaoning Huishan Holdings(Group)Co.LTD, Liaoning province, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 882-885

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）