The protoplast preparation of two strains of Saccharamyces cerevisae

摘要：

　　This paper researched on the condition of protoplast preparation for two strains isolated by myself,selected the different of time,concentration of zymolase and temperature,in the end,the proper condition was the 0.2mg/ml of zymolase,30℃,the time of 40 minutes,the ratio of regeneration was 68.2%.

关键词： Saccharamyces cerevisae protoplast protoplast preparation

作者: Chun hai Zhao Zhi gang Liu

作者单位: Bin Zhou Polytechnic, Key Laboratory of Agrobiotechnology of binzhou, 256603 ChinaShandong Binzhou Vocational College, Huanghe Road, No.919 Binzhou, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 898-901

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）