Purification and characterization of ginsenoside-hydrolyzing β-glucosidase from wheat bran

摘要：

　　The ginsenoside-hydrolyzing β-glucosidase that can converse the major ginsenosides into the minor ginsenosides was isolated from wheat bran,and the enzyme was purified and characterized.The crude enzyme solution extracted from wheat bran could hydrolyse the protopanaxadiol-type ginsenosides such as Rb1,Rc,Rd and Rg3,but could not hydrolyse the protopanaxatriol-type ginsenosides such as Re and Rg2.The enzyme fractionated on the DEAE-Cellulose DE-52 colunm was purified to one spot in SDS polyacrylamide gel electrophoresis,and the molecular weight of enzyme in the fraction 34,47,and 61 was approximately 62 kDa,62 kDa,and 68 kDa,respectively.

关键词： purification characterization β-glucosidase ginsenside-hydrolyzing wheat bran

作者: Chunzhi Zhang Ming Chen Haichen Guo Guoren Zu Li Chen

作者单位: College of Bioengineering, Dalian Polytechnic University, Dalian 116034, P.R.China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 906-909

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）