Characterization and Functional Expression of Xylose Isomerase from Thermus thermophilus

摘要：

　　Ethanol produced from hexose and pentose sugars hydrolysated by lignocellulose is an environment-friendly alternative to fossil fuels.Xylose isomerase is.the major rate-limiting enzyme in the ethanol synthesis biologically pathway of xylose fermentation.In present study,xy/A gene encoding xylose isomerase was cloned from Thermus thermophilus and overexpressed in E.coli BL21.Purified recombinant enzyme was used to study the enzymatic characterization.Specific activity of recombinant PDOR was 19.6 U/mg.Optimal temperature and pH were 80 ℃,8.0,respectively.Km and Vmax values were 15.9 mM,22.8 U/mg.This research may form a basis for the future application of xylose isomerase.

关键词： Thermus thermophilus xylA overexpression Characterization

作者: Angen Lu ZexiYang Fei Wang Lang Xu Wenying Deng Jianzong Meng Ziqi Huang Xianghui Qi

作者单位: Guangxi Zhuang Autonomous Region Center for Analysis and Test Research, Nanning, China;School of Foo School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China Guangxi Zhuang Autonomous Region Center for Analysis and Test Research, Nanning, China

会议类型: 国际会议

会议名称: 2012 The 2nd International Conference on Biotechnology,Chemial and Materials Engineering(第二届生物、化工和材料工程国际会议(CBCME2012))

会议地点: 厦门

会议语种:英文

页码: 919-922

在线出版日期: 2012-12-28（万方平台首次上网日期，不代表论文的发表时间）