Enzymological properties of magnetic chitosan microspere-immobilized alkaline protease

摘要：

　　Alkaline protease was immobilized with Magnetic chitosan microspheres compound with Fe3O4 and chitosan,glutaraldehyde as cross-linking agent.Watching and analysising its appearance and structural properties,the results showed that:it had good Spherical appearance,its size increasing from 15 to 20 nm after immobilized; Fe3O4 was surround by chitosan well analyzed with FTIR; it had full crystal structure and good ability of magnetic responsivenes and super magnetism before and after immobilization.The optimum pH of immobilized enzyme is 10,optimum temperature is 60℃,operational stability of the immobilized enzyme is better.Furthermore,the enzyme activity of immobilized enzyme is only reduced by 40% after used five cycles.

关键词： magnrtism chitosan immobilize alkaline protease

作者: Y.Li S.N.Wang L.Z.Jiang D.D.Li

作者单位: Food Collage, Northeast Agriculture University, Harbin, China

会议类型: 国际会议

会议名称: 2012 International Conference on Frontiers of Energy and Environmental Engineering(2012年第二届能源与环境工程前沿国际会议(ICFEEE2012))

会议地点: 香港

会议语种:英文

页码: 569-572

在线出版日期: 2012-12-01（万方平台首次上网日期，不代表论文的发表时间）