Lignin Biodegradation by Sphingobacterium.HY-H, Screening from Aerobic Sludge of Pulp and Paper Mill

摘要：

　　Six lignin degrading bacterial species,screening from activated sludge of pulp and paper mill,were isolated on mineral salt medium with lignin as the sole carbon and energy source.By comparing the results of the biodegradability tests,strain HY-H,identified as Sphingobacterium species by biochemical tests and 16SrRNA gene sequencing,was selected for subsequent tests on lignin biodegradation mechanism.The results showed that the strain HY-H was grow well under the condition:pH 7.0,temperature 30℃,and lignin/ammonium chloride ratio of 5.Under the optimum conditions,the maximum degradation capacity of the strain HY-H reached to 30%.GC-MS analysis indicated that after incubated with Sphingobacterium.HY-H,low molecular weight acids and lignin-related monomer compounds such as butyric acid,propylmalonic acid,acetovanillone were found,which strongly confirmed the degradation of lignin by Sphingobacterium.HY-H.

关键词： Lignin Sphingobacterium.HY-H aerobic bacteria biodegradation GC-MS

作者: Wenjing Du Jidong Liang Dongqi Wang

作者单位: Xian Jiaotong University,Xian,Shaanxi,China

会议类型: 国际会议

会议名称: 3rd International Conference on Pollution Ecology(第三届污染生态学国际会议(3rd ICPE))

会议地点: 天津

会议语种:英文

页码: 23-23

在线出版日期: 2012-11-06（万方平台首次上网日期，不代表论文的发表时间）