Study on the Effect of Copper Stress on MLH1, MSH2 Promoter Methylation of Arabidopsis Thaliana

摘要：

　　This paper adopts improved bisulfite DNA sequencing technique,combining with the morphological and physiological indices ofArabidopsis thaliana (Arabidopsis) seedlings,and studied the effects of Copper (Cu) stress on methylation of MLH1 and MSH2 promoters of mismatch repair genes of Arabidopsis thaliana seedlings,aimed to choose the biomarkers sensitive to Cu stress.The results showed that under Cu (0、0.5、3.0、5.0 mg·L-1)stress for 21 days,the root elongation growth was inhibited,but no significant changes were observed in leaf number and fresh weight of aerial parts.The total soluble protein content in shoots was decreased with increasing Cu concentration.The contents of chlorophylls increased at first and then decreased with the increasing Cu concentration,it displayed a negative correlation in total.

关键词： copper (Cu) MLH1 MSH2 biomaker promoter methylation Arabidopsis thaliana

作者: Ma Shanshan

作者单位: Institute of Applied Ecology,Chinese Academy of Science

会议类型: 国际会议

会议名称: 3rd International Conference on Pollution Ecology(第三届污染生态学国际会议(3rd ICPE))

会议地点: 天津

会议语种:英文

页码: 108-109

在线出版日期: 2012-11-06（万方平台首次上网日期，不代表论文的发表时间）