Response of alfalfa (Medicago sativa L.) bud seedling to saline and alkaline stress under water culture conditions
Bud seedlings were used to study short-term effect of different saline and alkaline concentrations on alfalfa root and stem growth.Two neutral salts (NaCl and Na2 SO4) and 2 alkaline salts (NaHCO3 and Na2CO3) were mixted at 9∶1 mole ratio,respectively,to imitate typical saline and alkaline environments.8 saline concentrations:0,10,20,40,60,80,100,120 mmol/L,and 7 alkaline concentrations:0,2,5,10,20,40,50 mmol/L were selected to determine the stress effects on 5-day,2-cm bud seedlings.The results indicated that bud seedlings root length increased firstly and then decreased as the increase of stress concentrations.On condition of neutral salt stress,40 mmol/L treatment showed optimum root length,which was 102% times higher than CK,with significant difference (P<0.05).Under alkaline salt stress,5 mmol/L treatment showed optimum root length,which was 156% times higher than the CK,with significant difference (P<0.05).The effects of the stresses on stem length surpassed root length.And the inhibitory effect of alkaline salt stress on seedlings growth in early stage surpassed neutral salt stress.
bud seedling, saline and alkaline stresses seedling growth water culture
Jianfeng Li Shuqing Zhang Shangli Shi Pinghui Huo
Key Laboratory for Grassland Ecosystem of Ministry of Education, College of Grassland Science, Gansu Agricultural University, Lanzhou, 730070, Peoples Republic of China;Sino-U.S.Centres for Grazingland Ecosystem Sustainability, Lanzhou, 730070, Peoples Republic of China
国际会议
石家庄
英文
33-37
2012-10-26(万方平台首次上网日期,不代表论文的发表时间)