Electrochemical aptasensor for label-free detection of protein based on gold nanoparticle involved self-assembly
A label-free electrochemical biosensing strategy based on gold nanoparticle involved layer-by-layer self assembly for the detection of protein is proposed using platelet derived growth factor-BB dimer (PDGF-BB) as the model analyte.Utilizing the strong sulfur-Au affinity,ethanthiol and capture probe modified gold nanoparticles are self-assembled onto the surface of gold electrode successively.The aptamer probe for target protein hybridizes with the capture probe and the biosensor is fabricated.By measuring ac current voltammetry,the target protein can be sensitively detected in a linear dynamic range from 1-1000 ng/mL with a low detection limit of 0.5 ng/mL.Making use of self-assembled gold nanoparticles layer,a large amount of capture probes can be modified onto the gold electrode,supporting the high sensitivity of the proposed strategy.In addition,good reproducibility,high selectivity and stability are achieved.In particular,the biosensor can be easily regenerated by melting in hot water,making it reusable.
PDGF-BB aptasensor gold nanoparticle self-assembly
Song-Bai Zhang Bing-Jun Zhang Qian-Liu Xia Hu Li-Ying Zheng Xue-Wen Liu Ji-Lin Lu Hui Zhou Shi-Biao Zhou
College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engine
国际会议
石家庄
英文
177-182
2012-10-26（万方平台首次上网日期，不代表论文的发表时间）