Relationships between Polarity, Aliphaticity/Aromaticity, Fluorescence and Molecular Size of Soil HA Electrophoretic Fractions
Reversed-phase ultra-performance liquid chromatography (RP-UPLC) with on-line absorbance and fluorescence detection was used for analysis of chernozem soil humic acids (HAs) and their fractions A,B and C+D with different electrophoretic mobility (EM) and molecular size (MS).A strong relationship between fluorescence properties,EM,MS,polarity and aliphaticity/aromaticity of HAs fractions was found.Humic and protein-like fluorescence had diffepent polarity nature.Protein-like fluorescence is located in humic material which irreversibly adsorbed on the reverse-phase column and was not subjected to RP-UPLC characterization.The humic-like fluorescence is mostly located in hydrophilic part of fraction C+D.Taking into account that high MS fraction A consists mainly of aliphatic components it is reasonable to suggest that these associations are capable of organizing into micellar structures.
Humic acids Polarity Aromaticity Fluorescence Molecular size
Olga Trubetskaya Claire Richard Guillaume Voyard Oleg Trubetskoj
Branch of Shemyakin and Ovchirnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences,14 Laboratoire de Photochimie Moléculaire et Macromoléculaire,UMR CNRS-Université Blaise Pascal 6505,63 Institute of Basic Biological Problems,Russian Academy of Sciences,142290,Pushchino,Moscow region,Ru
国际会议
the 16th Meeting of the International Humic Substances Society (第十六届国际腐殖物质学会大会(IHSS 16))
杭州
英文
105-107
2012-09-09（万方平台首次上网日期，不代表论文的发表时间）