Reaction Rates in Enzymatic Assay System in Solutions of Metal Salts and Humic Substances

摘要：

　　The paper considers the effects of humic substances (HS) on solutions of model inorganic pollutants,metal salts-K3Fe(CN)6 and CuSO4.Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H:FMN-oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presence and absence of HS.Ability of HS to decrease or increase general toxicity of the solutions was revealed.The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS.Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase.Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions.

关键词： Metal salts Humic substances Toxicity Bioluminescent assay Enzyme reaction rates

作者: Anna S.Tarasova Nadezhda S.Kudryasheva

作者单位: Siberian Federal University,Krasnoyarsk,660041,Russia Siberian Federal University,Krasnoyarsk,660041,Russia;Institute of Biophysics SB RAS,Krasnoyarsk,660

会议类型: 国际会议

会议名称: the 16th Meeting of the International Humic Substances Society (第十六届国际腐殖物质学会大会(IHSS 16))

会议地点: 杭州

会议语种:英文

页码: 315-317

在线出版日期: 2012-09-09（万方平台首次上网日期，不代表论文的发表时间）