Feasibility of Chelating Agent Utilization for Suppressing Methane Production during Soil Organic Matter Decomposition
Methanogens utilize simple carbon compounds to produce methane (CH-4) under strictly anaerobic conditions.During methanogenesis,methyl coenzyme M (MeCoM) is reduced by MeCoMreductase enzyme to CH4 involving a nickel-containing cofactor F430.In this experiment,astrong chelating agent,e ethylenediaminetetraacetic acid (EDTA),was applied in soil to study its feasibility for suppressing methanogen activity and CH4 production.Application of EDTA significantly (P≤0.05) reduced CH4 production.Applied EDTA forms complex compounds with nickel (Ni),the cofactor of Me-CoMreductase.Since methanogenesis is an intracellular process,it is necessary for methanogens to assi,milate those Ni-EDTA complexes as holoenzymesinside the cell to utilize Ni in EDTA treated soils.Results indicated that methanogens cannot utilize Ni in the presence of EDTA and that significantly (P≤0.05) reduced mcrA genes (coding MeCoMreductase enzyme) copy number and Co-M concentration in soil.Due to high correlations (r=0.901*) between Co-M concentration and mcrA gene copy numbers,Co-M concentrations could be used as an alternative biomarker for methanogens.Therefore,EDTA could be a good material to suppress CH4 emission from anaerobicsoil by restricting Ni availability to methanogens.
EDTA Ni availability Coenzyme M Methanogens CH4 production
Prabhat Pramanik Pil Joo Kim
Division of Applied Life Science,Gyeongsang National University,Jinju 660-701,Korea
国际会议
the 16th Meeting of the International Humic Substances Society (第十六届国际腐殖物质学会大会(IHSS 16))
杭州
英文
466-468
2012-09-09（万方平台首次上网日期，不代表论文的发表时间）