pH Dependence of Configurations and Surface Properties of Microbial Extracellular Polymeric Substances (EPS)

摘要：

　　The flocculability of microorganisms plays a crucial role in bioreactors,and is substantially affected by pH.However,the mechanism for such an effect remains unclear.In this work,the pH dependence of configuration and surface property of microbial EPS,excreted from Bacillus megaterium TF10,and accordingly its flocculability is elucidated.The main functional groups and buffering zones in the EPS were identified from FTIR and acid base titration.The laser light scattering analysis revealed that the deprotonated or protonated states of these functional groups in EPS were governed by pH and resulted in more dense and compact structure at a lower pH because of hydrophobicity and intermolecular hydrogen bonds.The zeta potential measurements implied that the isoelectric point and the electrostatic repulsion action of EPS were controlled by pH.These results clearly demonstrate that the EPS configurations and accordingly microbial flocculability are heavily dependent on pH in solution.

关键词： Extracellular polymeric substances (EPS) Flocculability Surface property Configuration pH dependent Laser light scattering

作者: Lingling Wang Xiangke Wang Longfei Wang Xuemei Ren Xiaodong Ye Wenwei Li Shijie Yuan Min Sun Guoping Sheng Hanqing Yu

作者单位: Department of Chemistry,University of Science & Technology of China,Hefei 230051,China Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230026,China Department of Chemical Physics,University of Science & Technology of China,Hefei 230051,China

会议类型: 国际会议

会议名称: the 16th Meeting of the International Humic Substances Society (第十六届国际腐殖物质学会大会(IHSS 16))

会议地点: 杭州

会议语种:英文

页码: 508-510

在线出版日期: 2012-09-09（万方平台首次上网日期，不代表论文的发表时间）