Response of community structure and activity of methanotroph to different CH4/O2 ratios
Biological methane oxidation is affected by many environmental factors.In this study,13C-labeling coupling with PLFAs detection was used to investigate the community structure of methanotrophs and the methane oxidation rate under different CH4/O2 ratios (1∶1,1∶3 and 1∶5 respectively).Results showed that variation of PLFAs concentration (ΔPLFAs) and carbon isotope richness (Δ13CPLFAs) of the soil incubated under the above CH4/ O2 ratios (CH4 concentration:15.6% 6.3% and 3.9% respectively) were both significant,and were positively related to the methane concentration.ΔPLFAs and ΔA13CPLFAs of 16∶1ω7c,16∶1ω5c and 16∶0 were the most significant for all the three different incubations.The rates of oxygen consuming/methane consuming under the three incubation conditions were 1.88,1.73 and 1.62 respectively,showing different assimilation occured.Methane oxidation rate per methanotrophic biomass calculated from 16∶1tω7c,16∶1ω5c,and 16∶0 were 1.11×10-7,1.99×10-7,1.53×10-7 nmol·h-1·cell-1 respectively.
PLFAs methanotroph 13C-labeling methane oxidifion
Lulu Ren Huizhi Li Wenjing Lu
School of Environment, Tsinghua University, Bejing, China, 100084
国际会议
The Seventh International Conference on Waste Management and Technology(第七届固体废物管理与技术国际会议(ICWMT7))
北京
英文
697-704
2012-09-05(万方平台首次上网日期,不代表论文的发表时间)