MEASUREMENT OF TRANSPORT IN RENAL CELL SUSPENSIONS DURING FREEZING
Understanding the biophysical processes that govern freezing responds of cells is an important step in characterizing and improving the cryopreservation.The quantitative analysis on cell volume shrinkage during freezing helps us understand the mechanism of cryopreservation.Freezing studies were conducted using a Linkam cryostage fitted to an optical microscope cooled under controlled rates at 5,10 and 20℃/min.The volume of renal cell at subzero temperature have been quantified by heat latent obtained from DSC data and compared to the microscopic data.Experimental data were fitted by nonlinear regression method to calculate the water transport parameters.
Zhu K Wang Y.B An N Wang Y.J
Tianjin Key Laboratory of Refrigeration Technology Tianjin University of Commerce,Tianjin University Department of Thermal Engineering,School of Environmental Science and Engineering,Tianjin University
国际会议
西安
英文
1-8
2012-08-26（万方平台首次上网日期，不代表论文的发表时间）