A Novel Pipeline for Motif Discovery, Pruning and Validation in Promoter Sequences of Human Tissue Specific Genes

摘要：

　　Identification and analysis of tissue-specific (TS) genes and their regulatory activities play an important role in the understanding of mechanisms of organisms,disease diagnosis and drug design.In this paper,we designed a pipeline for the discovery of promoter motifs for tissue-specific genes.The pipeline consists of three phases: motif searching,motif merging and motif validation.The motif searching phase integrated three algorithms: MEME,AlignACE and Gibbs Sampling.In the second phase,we proposed a motif merging method,which is based on Bayesian probabilistic principles,to reduce redundancies of motifs from the first phase.Lastly,the motif validation phase verified the statistical significance of discovered motifs using a Bayesian Hypothesis Test approach.We performed the analysis on the sequences of promoter regions (-449bp-1000bp) of 4,552 human tissue-specific genes across 82 tissues and 924 housekeeping genes.The distributions of motifs in different promoter regions show that most motifs prefer to be in the proximal region (+500~50bp,-50bp~-500bp) of promoters.

关键词： tissue specificity motif discovery Bayesian hypothesis test promtoer sequences

作者: Xiu-Jun GONG Hua YU Fei-Fei ZHAO

作者单位: School of Computer Science and Technology Tianjin UniversityTianjin, China State Key Laboratory of Management and Control for Complex SystemsInstitute of Automation, Chinese A

会议类型: 国际会议

会议名称: 6th International Conference on Systems Biology (第六届国际系统生物学会议)(ISB2012)

会议地点: 西安

会议语种:英文

页码: 43-48

在线出版日期: 2012-08-19（万方平台首次上网日期，不代表论文的发表时间）