Optimization of Electroporation Processing of Bacillus subtilis

摘要：

　　In order to obtain an optimal transformation efficiency of Bacillus subtilis electroporation experimental program,to optimize the conditions for electroporation of Bacillus subtilis cells,a shuttle vector for Escherichia coli and Bacillus subtilis,pHY300PLK,were used.Factors including growth medium,electroporation medium,recovery Medium,plasmid concentration and pulse strength were investigated.Single factor experiments were employed to optimize the growth medium,electroporation medium,recovery Medium,plasmid concentration and pulse strength.Results showed that the optimal values for concentration of the growth medium electroporation medium,recovery Medium,plasmid concentration and pulse strength are LB+0.5 M sorbitol,0.5 M sorbitol+0.5 M mannitol+10% Glycero,LB+0.5 M sorbitol+0.38 M mannitol,20 kV/ cm and 100 ng/μl ofPlasmid DNA.

关键词： Bacillus subtilis Electroporation Transformation efficiency

作者: Jinbin Wang Dachao Chen Ziping Zhu Ying Xu Xueming Tang

作者单位: Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai, Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai, Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,2901 Beidi Road,Shanghai,

会议类型: 国际会议

会议名称: Conference on Environmental Pollution and Public Health (2012年环境污染与大众健康学术会议(CEPPH2012))

会议地点: 上海

会议语种:英文

页码: 679-683

在线出版日期: 2012-08-09（万方平台首次上网日期，不代表论文的发表时间）