Cellular uptake of PEGylated PLGA nanoparticles in Hela cells

摘要：

　　PLGA nanoparticles (PLGA-NPs) are being extensively studied as drug carriers for their controlled release,biodegradability and biocompatibility.This study evaluated the cellular uptake of PEGylated PLGA-NPs in Hela cells.MePEG-PLGA (5%-15%) was used to prepare PEG modified PLGA nanoparticles (PEG-PLGA-NPs),and the fluorescent marker DiI was encapsulated in the nanoparticles for the visualized analysis.The nanoparticles were characterized for surface morphology,particle size,zeta potential,and for cellular uptake by Hela cells.Results showed that PLGA nanoparticles were lowly cytotoxic and could be uptaken by Hela cells freely.PEG-PLGA-NPs had faster cellular uptake than that of nude PLGA nanoparticles,especially 10%PEG-PLGA-NPs.It suggested that the surface modification of PLGA-NPs by PEG notably improved the cellular uptake.

关键词： PLGA nanoparticles PEG Dil Hela cells Cellular uptake

作者: Gang Li Donghai Lin Xinxin Xie Lifang Qin Junteng Wang

作者单位: Life School of Yantai University, Yantai 264005, PR China Pharmacy School of Yantai University, Yantai 264005, PR China

会议类型: 国际会议

会议名称: the 2012 International Conference on Frontiers of Nanoscience and Technology(2012年纳米科学与技术国际会议(ICFNST 2012))

会议地点: 香港

会议语种:英文

页码: 80-83

在线出版日期: 2012-07-26（万方平台首次上网日期，不代表论文的发表时间）