Establishment of a Regeneration System for the Genetic Transformation of Ground-Cover Chrysanthemum Jindinghongxin
Ground-cover Chrysanthemum has been used in gardens due to its rich colors,excellent ornamental value.Too much precipitation in summer of north China limits its further use.In order to create chrysanthemum with high resistance to waterlogging, the Vgb (Vitreoscilla hemoglobin) and pmi (phosphomannose isomerase)genes are transferred into the genome of ground-cover chrysanthemum Jindinghongxin by Agrobacterium tumefaciens mediated transformation in our research.This research is of profound importance to broaden the usage of ground-cover chrysanthemum in gardens.This research optimized the regeneration system of Jindinghongxinincluding choosing young leaves as explants and the best regeneration culture medium (MS+2.0 mg/L 6-BA+0.5 mg/L NAA), Transformation system for Jindinghongxin was established by pre-culturing leaf explants for 2 days.The suitable concentration of Agrobacterium tumefaciens and the suitable dipping time for transformation was OD600=0.4 for 5 minutes.Co-cultivation was done for 2 days at 28℃ in the dark after infection and the explants were then put in delayed culture for 4 days.Then the explants were cultured in first selection medium with 6 g/L mannose for 15 days, and then cultured in second selection medium with 7 g/L mannose.Once the regeneration shoot was 1 cm long it was transformed into MS with 350 mg/L carbenicillin for root induction.
ground-cover Chrysanthemum regeneration system optimization transformation system water tolerant
Y.Wang W.Z.Liu Y.K.Gao
College of Ornamental Horticulture and Landscape Architecture Beijing Forestry University 35 Qinghua East Road, Haidian District, Beijing 100083 China
国际会议
International Conference on Germplasm of Ornamentals(国际观赏植物种植资源学术研讨会)
北京
英文
213-218
2012-07-16（万方平台首次上网日期，不代表论文的发表时间）