Adventitious Shoot Organogenesis and Plant Regeneration by Immature Zygotic Embryos of Osmanthusfragrans
Sweet osmanthus (Osmanthus fragrans Lout.), an evergreen shrub of Oleaceae family, is well known as an ornamental and economic plant for unique fragrance.The establishment of an efficient regeneration system and transformation system in sweet osmanthus for genetic improvement has not yet been reported.In this study, we firstly evaluated the effect of growth regulators on plant regeneration via organogenesis of sweet osmanthus.Immature zygotic embryos at cotyledonary stage were cultured on media with different concentrations of auxins and cytokinins.The results showed that the highest frequency of adventitious shoot regeneration (30%) was found in the 3.0 mg/L 6-BA+0.5 mg/L TDZ+0.5 mg/L NAA after 6 weeks dark treatment, and then shoots grew and prolonged in MS medium after another 6 weeks under light.The regenerates rooted when in the medium containing MS+2.0 mg/L NAA or MS basal medium and developed successfully after two months.
Osmanthusfragrans plant regeneration zygotic embryos organogenesis
J.J.Zou W.Gao X.Cai C.Y.Wang
Key Laboratory for Biology of Horticultural Plants Ministry of Education College of Horticulture & Forestry Sciences Huazhong Agricultural University Wuhan 430070 China
国际会议
International Conference on Germplasm of Ornamentals(国际观赏植物种植资源学术研讨会)
北京
英文
353-357
2012-07-16（万方平台首次上网日期，不代表论文的发表时间）