Tissue Culture and Regeneration of Paeonia suffruticosa Andr.
This paper was to study tissue culture technology of a Chinese tree peony Luo Yang Hong, and established in vitro regeneration system initially.With the combination of different kinds of treatments, the effects of basic medium, plant growth regulators, and some other important factors on shoots multiplication, root induction, callus induction and differentiation were studied.The main results showed as follows: the optimum multiplication medium of peony Luo Yang Hongwas PM + 0.5-1.5 mg·L-1 6-BA + 25 g·L-1 sucrose, rooting medium was 1/2PM +15.0 mg·L-1 IBA + 30 g·L-1 sucrose, callus induction medium from the petiole as the explant was PM + 2.0~3.0 mg.L-1 6-BA + 4.0 mg.L-1 2,4-D + 0.1-0.5 mg·L-1 NAA +30 g·L-1 sucrose, callus differentiation medium was PM + 2.0 mg·L-1 6-BA +1.0 mg·L-1 2,4-D + 500 mg·L-1 LH or CH + 0.01 mg·L-1 TDZ + 45 g·L-1 sucrose.
Paeonia suffruticosa Andr. tissue culture regeneration
J.Q.Zhang X.N.Jiang X.F.Lu
Public Analysis and Test Center of Beijing Forestry University Beijing China College of Biological Sciences and Biotechnology of Beijing Forestry University Beijing China
国际会议
International Conference on Germplasm of Ornamentals(国际观赏植物种植资源学术研讨会)
北京
英文
399-405
2012-07-16（万方平台首次上网日期，不代表论文的发表时间）