Effect of Antifouling Dendrimers and Dendrimer-Entrapped Gold Nanoparticles on the Improvement of Polymerase Chain Reactions

摘要：

　　The polymerase chain reaction (PCR) has become a fundamental technique in molecular biology.However,the specificity of PCR does not always match its remarkable sensitivity.Development of suitable PCR additives to enhance the PCR efficiency and specificity and understanding the enhancing mechanism still remain great challenges.We report the use of generation 5 (G5) poly (amidoamine) (PAMAM) dendrimer and dendrimer-entrapped gold nanoparticles (Au DENPs) modified with polyethylene glycol (PEG) moieties and/or acetyl groups as a novel class of enhancers to improve the PCR specificity and efficiency.We show that dendrimers with the entrapment of Au NPs seem to more effectively to enhance the PCR specificity and efficiency.In addition,the modification of antifouling PEG significantly lowers the optimization capability of the corresponding dendrimers,while this inhibition effect can be compromised by the entrapment of Au NPs.The developed dendrimer derivatives or nanocomposites may be used as highly efficient PCR additives for enhancing different PCR systems.

关键词： Polymerase chain reaction PEGylation Dendrimers Au nanoparticles Specificity Efficiency

作者: ZHANG Hai-xia CAO Xue-yan SHI Xiang-yang PENG Chen

作者单位: College of Chemistry, Chemical Engineering and Biotechnology , Donghua University, Shanghai , China College of Materials Science and Engineering, Donghua University, Shanghai , China

会议类型: 国际会议

会议名称: 2012 International Forum on Biomedical Textile Materials (2012国际纺织生物医用材料论坛)

会议地点: 上海

会议语种:英文

页码: 68-72

在线出版日期: 2012-06-08（万方平台首次上网日期，不代表论文的发表时间）