Study of Collagen Assemble into Gel in Situ Used as Tissue Engineering Scaffold
Collagen is a kind of protein which can self-assemble into collagen gel in vitro at 37 ℃ in neutral pH.In this work,collagen is extracted from bovine tendon and characterized by amino acid analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).When pH of collagen solution is adjusted to 6.6~6.8 and incubated at 37℃ for an hour,collagen molecular can be assembling into gel.As a result,collagen fibers in gel observed form electron microscopic (SEM and TEM) look like natural collagen fiber with cross-grain,which is caused by collage molecular assembly behavior.Then mouse embryonic fibroblasts (3T3 cells) are seeded in the gel.Within a period,state of 3T3 cell living in the 3D gel scaffold is observed.As a result,when collagen solution is mixed with 3T3 cells and incubated in vitro,the gel is formed and provides a three-dimensional space for 3T3 cells proliferation at the same time.This study provides a method to make tissue engineering scaffold with cells in situ and predicts its application in soft tissue defect healing.
collagen gel self-assemble scaffold
Yuan Cui Yanhui Li Qian Duan
Full address of first author,including country School of Materials Science and Technology,Changchun University of Science and Technology,Chanachun,China
国际会议
太原
英文
146-151
2012-01-13（万方平台首次上网日期，不代表论文的发表时间）