SPOUTED BED DRYING AS A METHOD FOR ENZYME IMMOBILIZATION
Immobilization is usually a requirement to the use of enzymes as an industrial biocatalyst.In this work,endophytic fungus Cercospora kikuchii lipase was immobilized by covalent binding on agricultural by-products and microcrystalline cellulose.The enzyme-support system was dried in spouted bed.Microcrystalline cellulose with 1.5% of glutaraldehyde showed the best support,maintaining 179.1% of activity after drying,followed by the rice straw (173.9%),corn stover (169.8%),sugarcane bagasse (157.3%),green coconut fiber (102.3%) and corn cob (99.8%).The use of these immobilized derivatives obtained after spouted bed drying may help to increase the productivity while minimizing product recovery cost.
spouted bed enzyme immobilization lipase Cercospora kikuchii agricultural by-products
T.A.Costa-Silva R.C.Cognette C.R.F.Souza S.Said W.P.Oliveira
University of S(a)o Paulo,Faculty of Pharmaceutical Sciences of Ribeir(a)o Preto Av.do Café s/n,Ribeir(a)o Preto,P.O.B.14040901,Brazil
国际会议
The 18th International Drying Symposium Conference(第十八届国际干燥学术大会(IDS2012))
厦门
英文
1-7
2012-01-11(万方平台首次上网日期,不代表论文的发表时间)