cDNA Cloning and Sequence Analysis of a Heat Shock Protein Gene,LsHsp90, from Leaf Lettuce (Lactuca sativa L.)

摘要：

　　In this study,a heat shock protein gene was isolated from the leaf lettuce (Lactuca sativa L.) by using Reverse transcription-polymerase chain reaction (RT-PCR) and Rapid-amplification of cDNA ends (RACE) approaches,and the gene was named LsHsp90.The full length cDNA of LsHsp90 is 2330 bp,with an Open reading frames (ORF) of 2097bp,encoding 698 putative amino acid residues with a predicted molecular mass of 79.8 kD.Homology analysis showed that the deduced HSP90 protein was highly homologous to other HSP90 proteins from different species.Phylogenetic analysis among 13 species indicated that LsHSP90 was clustered with the HSP90 from Ageratina adenophora.

关键词： Lattuca sative L. heat shock protein gene cloning sequence analysis

作者: Yue Ren Yingyan Han Shuangxi Fan Ting Li

作者单位: Plant Science and Technology College,Beijing University of Agriculture/New Technological Laboratory in Agriculture Application in Beijing,Beijing 102206,China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 91-97

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）