Construction of Plant Expression Vectors about the SERK Gene of Pingyi Tiancha and its Crossbred Offspring
In order to explore the molecular mechanism about the effect of SERK gene on the apomixis in Pingyi Tiancha (Malus hupehensis var.pingyiensis) and its crossbred progenies,the ovary cDNA of Pingyi Tiancha and its crossbred offspring No.33 strain were used as the templates,and SERK1 gene was amplified by polymerase chain reaction (PCR).SERK1 gene was then cloned into plant expression vector pBI121 and its plant expression vectors were obtained,which were pBI121-MhSERK1 and pBI121-MhdSERK1.DNA sequencing showed that pBI121-MhSERK1 and pBI 121-MhdSERK1 vectors had been constructed successfully,and could be used in the subsequent transgenic research.
SERK gene expression vector Malus
Zhang Lijie Wang Yuxia Dong Wenxuan
College of Horticulture,Shenyang Agricultural University
国际会议
The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))
北京
英文
110-113
2012-11-24（万方平台首次上网日期，不代表论文的发表时间）