A Method of Mitochondrial DNA Isolation in Peach
Mitochondrial DNA is widely used in the studying field of mechanism of cytoplasmic male sterility,heredity breeding of plant.Based on the former researches and field experiences of breeding researchers,the extrachromosomal inheritance has shown remarkable influence on peach breeding.In order to study on germplasm identification and germplasm resources research via peach extrachromosomal inheritance,a repmducible,simple and convenient mitoehondrial DNA isolation method is expected.This study investigated exacting mitochondria and exacting and purifying mtDNA from fresh leaves of peach,respectively.The result indicated that after the high speed tissue machine,high purity mitochondria could be exacted by differential centrifugation and sucrose density gradient centrifugation; finally,the process is preliminarily obtained,which can satisfy normal experiment requirements.
Peach Mitochondria Isolation Mitochondrial DNA (mtDNA)
Zhao Huimin Song Liqin Yu Fengming Zhang Libin
Hebei Normal University of Science & Technology,Qinhuangdao,Hebei 066604,China
国际会议
The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))
北京
英文
197-200
2012-11-24（万方平台首次上网日期，不代表论文的发表时间）