Selection of Reference Gene for Real-time Fluorescence Quantitative PCR in Dahlia Petals

摘要：

　　In order to accurately and quantitatively analyze expression characteristics of senescence-associated genes in dahlia petal,the expression stability of three reference genes (18S rRNA,β-actin and GAPDH) was studied through real-time fluorescence quantitative PCR (qRT-PCR) under the different growth phases of dahlia petal,including squaring period,early florescence,full-bloom stage and flower senescence stage.The results show that the RT-PCR products are entirely consistent with the expected size.GeNorm and NormFinder analyses reveal that β-actin is the relatively more stable reference gene,and can be used for qRT-PCR of DpXTH gene expression related to petal senescence in dahlia.

关键词： Dahlia real-time fluorescence quantitative PCR reference gene DpXTH gene gene expression

作者: Wang Lei Yan Junfang Wang Jing Tan Jianzhong

作者单位: Department of Horticulture,School of Architecture and Urban Environment,Soochow University,Suzhou 215123,China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 216-219

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）