In Vitro High Frequency Direct Plant Regeneration from Leaflet of Blackberry cv.‘Kiowa’
An efficient protocol for direct regenerationof blackberry (Rubus hybrid) cv.‘K iowa (PI No.638183) from immature leaflet explants excised from axenic seedlings has been developed.Initially,the leaflets from 5-week-old mother cultures on MS medium were used as the explants.Treatments on MS medium with 21 days dark treatment and from the 1st (from top) leaflets revealed better regeneration effects.Cultures on medium MS+ forchlorfenuron (CPPU) 1.0 mg·l-1+indole-3-acetic acid (IAA) 0.5 mg·l-1 produced the highest regeneration frequency (95.00%); The best proliferation medium (coefficient 4.70) was MS+ N6-benzylaminopurine (BAP) 0.5 mg·l-1 +IAA 0.1 rag·1-1.The optimum rooting medium was 1/2 MS+a-naphthalene acetic acid (NAA) 0.03 mg· 1-1.The results were valuable for rapid propagation and genetic transformation of blackberry.
Adventitious bud Blackberry Differentiation Leaflet Regeneration Rubus Tissue culture
Chunhong Zhang Lubei Wang Wenlong Wu Weilin Li
Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China
国际会议
The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))
北京
英文
237-241
2012-11-24（万方平台首次上网日期，不代表论文的发表时间）