Optimization of Two-dimensional Electrophoresis for Proteome of Cucumber Cotyledon

摘要：

　　2-DE analysis of complex plant proteomes has limited dynamic resolution because only abundant proteins can be detected.In order to improve the detection rate of cucumber cotyledon low-abundance proteins for 2-DE,we report two different fractionation protocols for preparation of whole-cell proteins,differential polyethylene glycol (PEG) precipitation method and differential urea concentration method.After 2-DE and PDQuest analysis,one time more protein spots can be detected after each fraction method and Rubisco is enriched in one ffraction.The extraction and fractionation techniques are simple and versatile and can be used to visualize more proteins in 2-DE analysis of plant proteome.Our studies suggest successful depletion of Rubisco can be achieved using a similar fractionation protocol.

关键词： cucumber 2-DE PEG urea fraction

作者: YU Guang-chao HAO Yu-han Meng Xiang-nan FAN Hai-yan

作者单位: College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China;Key L

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 250-253

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）