Effect of Calcium on Ethylene Biosynthesis and Related Gene Expression in Grafted Muskmelon (Cucumis melo L.) Fruits
In this research,taking ‘New Seoul Tsui honey muskmelon (Cucumis melo) as scions and the white seed pumpkin (Cucurbita moschata Dueh.) ‘Shengzhen No.1 as rootstock,the paper aimed to study the effects of Calcium on ethylene biosynthesis in grafted muskmelon fruits,which used self-rooted plants as control and calcium nitrate to spray fruit surface as the treatment.The results showed that calcium treatment and grafting distinctly reduced concentration of ethylene,ACC content,ACC oxidase and ACC synthase enzyme activity,at the same time,they also decreased the relative gene expression level of the Cm-ACO1,Cm-ACO3,Cm-ACS1 and Cm-ACS2,especially,they also both let relative expression peak time of the Cm-ACS1 and Cm-ACS2 appear three days in advance.Besides,the grafting inhibited relative expression level of Cm-ACO2 throughout the maturity process,while calcium treatment only inhibited relative expression level of Cm-ACO2 in the late fruit development.On the other hand,the grafting improved expression of Cm-ACS3 gene transcription level in flesh of fruit late (after 41 days),unfortunately calcium treatment can inhibit the increase of the expression of this gene.
Calcium muskmelon ethylene ACC ACC synthase ACC oxidase gene expression
QI Hong-Yan DENG Chuan-Yu XU Chuan-Qiang ZHANG Yan-Fang
College of Horticulture,Shenyang Agricultural University/Key Laboratory of Protected Horticulture,Ministry of Education/Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang 110866
国际会议
The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))
北京
英文
258-262
2012-11-24（万方平台首次上网日期，不代表论文的发表时间）