Characterization of a Novel Glycerol-3-Phosphate Acyltransferase from Lilium dauricum and Evolution Analysis with Those from Other Plants

摘要：

　　The glycerol-3-phosphate acyltransferase genes (GATPs) are a kind of genes related with cold resistance of plants,which can be expressed by cold stress.A new Li-GAPT gene was cloned from Lilium dauricum in this research.Using Leaves as materials,a glycerol-3-Phosphate (GPAT) gene was cloned from the tissue culture regeneration plantlets of Lilium dauricum by the method of RT-PCR and rapid amplification of cDNA ends,and sequence analysis and homology comparison of the cDNA were performed by using DNArnan software and BLASTN program.The cloned full-length cDNA was 1544bp with 5-UTR of 10bp,an open reading frame of 1233bp,3-UTR of 31l bp and poly (A) tail of 19bp.The cDNA encoded 410 amino acids,and had a molecular size of 46 KDa.Its predicted that the differences between the 43 and 359 amino acid residues had a relationship with the differences of cold-stress in Lilium.The nucleic acid sequence analysis showed that it shared high homology with other known GPATs.

关键词： Lilium dauricum GPAT Cold stress bioinformatics analysis prokaryotic expression

作者: Chen Lijing Xiang Hengzuo Wang Li Li Tianlai

作者单位: Bioscience and Technology institute of Shenyang Agriculture University,Key Laboratory of Agriculture Bioscience and Technology institute of Shenyang Agriculture University,Key Laboratory of Agriculture Key Laboratory of Protected Horticulture,Shenyang Agricultural University,Ministry of Education,Dong

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 314-319

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）