Changes of Photosynthetic Pigments and Proteins from the Leaf Color Mutant in Mulberry

摘要：

　　To analyze the physiological and biochemical variation between normal and leaf color mutant (Cty-Sm)in mulberry,the photosynthetic pigments were extracted from the leaves of the leaf color mutant and control mulberry and the photosynthetic pigments contents were determined,then the protein were extracted and analyzed through MS/MS.The results showed that the contents of chlorophyll a,b and carotenoid in mutant leaves decreased significantly; the chlorophyll a was only 5.4~10.2% of the controls,which directly led to the leaf etiolation,meanwhile the proteins of the leaves were isolated through Gel electrophoresis and identified by mass spectrum analysis technology.16 differential expressed protein bands were detected,and the contents of large and small subunits of Rubisco and LHC Ⅱ decreased,which suggested that the leaf etiolation was related to the photosynthetic carbon assimilation and differential expression proteins of chlorophyll.

关键词： mulberry leaf color mutant photosynthetic pigment differential proteome SDS-PAGE

作者: Yin Peifeng Jia Junli Zheng Biping Tan Jianzhong

作者单位: Department of Horticulture,School of Architecture and Urban Environment,Soochow University,Suzhou,215123 China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 337-340

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）