Optimization of AFLP System for Tomatoes Based on Orthogonal Design

摘要：

　　Two inbred lines of tomato (Lycopersicon esculentum Mill.) FL1 and MFK1 with obviously different contents of lycopene were used in this experiment.In order to explore the amount of DNA,digested and connected time and pre-amplification product of dilution times,optimization of AFLP markers system were analyzed on the amplification results of the AFLP system The results showed that digested and connected time and the amount of DNA have the greatest impact on the amplification of the AFLP markers,and digested and connected time is the most critical factor for the PCR amplification results of this test.AFLP reaction system is established for tomatoes:300 ng of DNA template,6 h of digested time,pre-amplification products be diluted 40-fold.Bands amplified were stable and clear,so which can be applied to the tomato genetic map construction and tomato genetic diversity analysis.

关键词： Tomato AFLP Orthogonal design System optimization

作者: Jiang Jing Liu Yingming Li Kailong Zhang Aihua

作者单位: College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 375-378

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）