Regeneration of Leaf Culture in vitro in Lavandula angustifolia

摘要：

　　The leaves derived from tube plantlets of Lavandula angustifolia cv.‘Hidcote were applied as explants to culture on MS media supplemented with different concentrations of 0.1,0.5,1.0 mg·L-1KT and 0.5,1.0,2.0 mg·L-1 6-BA,0.1,0.5,1.0,2.0 mg·L-1 NAA and 0.1,0.5 mg·L-1 IBA to obtain the optimal media of callus induction,differentiation and proliferation.The results indicated that the optimal media of callus induction were MS + NAA 1.0 mg·L-1+ 6-BA 1.0 mg·L-1 and MS + NAA 0.5 mg·L-1 + KT 0.5 mg·L-1 with the induction rates of 96.2% and 93.5% respectively.The best medium for adventitious bud differentiation was MS + NAA 0.5 mg·L-1 + 2,4-D 6.0 mg·L-1 with the differentiation rate of 31.8%.The most suitable medium for plantlet multiplication was MS + NAA 0.5 mg·L-1 + 2,4-D 6.0 mg·L-1 with the shoot proliferation coefficient of 5.6 and plantlets grew strongly on it.

关键词： Lavandula angustifolia Leaf In vitro culture Regeneration

作者: Lei Jia Jun Xu Chuan Jun

作者单位: College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 432-435

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）