Isolation of a Male Sterile Gene (BrS1F) from Chinese Cabbage (Brassica rapa L.ssp.pekinensis)

摘要：

　　A different-expressed gene BrS1F between fertile and sterile buds was found from the male sterile A/B line ‘AB01 of Chinese cabbage (Brassica rapa L.ssp.pekinensis) by cDNA-AFLP (cDNA amplified fragment length polymorphism) method.RT-PCR (reverse transcription PCR) analysis shows that the housekeeping gene Actin had the same expression level between fertile and sterile buds,but BrS1F is a fertile-related gene as it is expressed only in fertile plants,but not in sterile plants.Moreover,Sequencing and NCBI Blast analysis showed that BrS1F is 326 bp and has 65% homology to the self-incompatibility family protein of Arabidopsis thaliana.But the exactly function of BrS1F should be confirmed further more.It lays the foundation for further cloning of the genes and revealing the molecular mechanism of genic multiple-allele-in-herited male sterility of Chinese cabbage.

关键词： Chinese cabbage (Brassica rapa L.ssp.chinensis) Male sterility cDNA-AFLP RT-PCR real-time

作者: Song Qian Feng Hui Xing Miaomiao Ji Ruiqin

作者单位: Department of Horticulture,Shenyang Agricultural University,Shenyang 110866,China

会议类型: 国际会议

会议名称: The Third Conference on Horticulture Science and Technology (2012第三届园艺科学与技术学术会议(CHST2012))

会议地点: 北京

会议语种:英文

页码: 445-448

在线出版日期: 2012-11-24（万方平台首次上网日期，不代表论文的发表时间）