In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells

摘要：

　　Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-1-(2,3-didodecylcarbamoyloxy)propyl-N,N,N-trimethylammonium iodide) as gene delivery carriers was described in this work.The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE.In addition,the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents,Lipofectamine2000 and DOTAP.The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.

关键词： cationic lipids DNA condensation gene delivery transfection efficiency

作者: Defu Zhi Shuibao Zhang Yinan Zhao Shaohui Cui Bing Wang Huiying Chen Defu Zhi Defeng Zhao

作者单位: SEAC-ME Key Laboratory of Biochemistry Engineering,Dalian Nationalities University,Dalian, China State Key Laboratory of Fine Chemicals, Dalian University of Technology,Dalian, China

会议类型: 国际会议

会议名称: 2012年国际材料与化学工程研讨会

会议地点: 北京

会议语种:英文

页码: 229-231

在线出版日期: 2012-10-26（万方平台首次上网日期，不代表论文的发表时间）